Category: News

We are very proud to share that GUF team was awarded 3rd place at the Goethe Innovation Prize Event with “Modulux3D” Project: a 3D bioprinter that revolutionizes medical research through tissue engineering. This award recognizes research projects with great innovation potential and benefits for customers or patients.

Last December 11 took place in Frankfurt a pitch competition to select the winning teams of this year’s Goethe Innovation Prize. This prize is conceded by Goethe-Unibator, the start-up center of the Goethe University Frankfurt am Main operated by the technology transfer company Innovectis. It provides students, academic staff, and alumni with active support for their start-up projects and acts as a bridge between science and business.

From a high number of applications, five projects qualified for the final and had the chance to deliver their pitches in 4 minutes in front of an audience of over 100 people. The three best teams were selected by an expert jury and won prize money and participation in the Unibator start-up program.

Louise Breideband and Levin Hafa presented the “Modulux3D” start-up, originated from B-BRIGHTER project, that aims to bring our 3D bioprinting technology to the market. They highlighted its innovative bio-printer that uses light to network biological cells and a special bio-ink. After a great and convincing pitch about the vast potential of 3Dbioprinting in the field of biomedicine, they won the third prize. Congratulations!

Last 16 and 17 of November, B-BRIGHTER members met in Frankfurt for the 12M project meeting at GUF premisses. They took the opportunity to see, first hand, the 3D bioprinter prototype.

GUF hosted the 12M B-BRIGHTER meeting, and researchers could discuss about the last advances of the project and define the future steps. On the first day took place the progress meeting, where the coordinator of each one of the 6 work packages presented their results to monitor the evolution of the project. Moreover, a closed discussion with the project officer and some invited experts took place, to improve the project and foster its outcomes. This discussion was highly productive as the project coordinator, Gustaf Mårtensson, outlined the goals, the product, the development program, and the current status of the project. He emphasized the achievements made during this first year and highlighted the plans for the next months.

Taking advantage of the meeting’s location in Frankfurt, a hands-on activity was scheduled so that researchers could get a close look at the 3D bioprinter prototype and do some small experiments to see it in use.

It was a very fruitful meeting!

The Cellink Partnership Conference took place from 25th to 27th of October in Portsmouth in the UK, and Levin Hafa from GUF presented a poster showcasing the last advances of the project.

For 2 days Portsmouth received scientists, leaders and experts to foster a growing community of collaboration and knowledge around bioprinting. The fifth edition of Cellink Partnership Conference aimed to provide participants with invaluable insights, showcase groundbreaking discoveries, network with peers, and celebrate advancements in the bioprinting field. The meeting brought together key opinion leaders, life science experts, and industries to share experiences on how bioprinting is being employed to address research challenges across various application areas. Some topics addressed were 3D Cell Culture, Personalized Medicine, Drug Development, Clinical Translation, Biomaterials, Alternatives to Animal Models and Tissue Engineering.

As it could not be otherwise, B-BRIGHTER was present at the conference. Levin Hafa from GUF presented a poster titled “Bioprinting by light sheet lithography: engineering complex tissues with high resolution at high speed”, with the participation of researchers from, GUF and IBEC. The poster highlighted the new B-BRIGHTER technology, a patented one-photon bioprinting technique that produces millimeter-sized objects in less than 3 minutes using a static light sheet and a digitally scanned perpendicular light beam and allows live acquisition of the printing process in high resolution for real-time observation.

The diverse sessions on the conference agenda presented a unique opportunity to learn about the current uses of bioprinting technologies, explore emerging trends and solutions, acquire practical knowledge through interactive workshops, and connect with the bioprinting community.

B-BRIGHTER researchers from GUF and IBEC publish an outstanding paper in Advanced Materials Journal presenting LUMINATE (Light sheet-based Ultrafast Microscopic Non-contact And Three-dimensional Enhanced bioprinting), a method that encompasses high printing speed and high resolution while introducing a fully integrated and streamlined fluorescent light sheet microscope capable of capturing real 3D images by optical sectioning the bioprinted construct.

Recently, a great work summarizing the research advances of B-BRIGHTER project came to light and was published in the prestigious journal Advanced Materials. The work, first-authored by Levin Hafa and signed by GUF and IBEC teams, shows that B-BRIGHTER’s innovative approach lies in its ability to combine high printing speed (0.66 mm³/s) with exceptional resolution (9 µm) using light sheet-based imaging.

The methodology involves direct laser patterning and a static light sheet for confined voxel crosslinking in photocrosslinkable materials. This not only enhances the precision of the bioprinting process but also allows for real-time monitoring of hydrogel crosslinking through techniques such as fluorescent recovery after photobleaching (FRAP) and brightfield imaging.

The versatility of the integrated system is demonstrated through the encapsulation of human fibroblasts in a thiol-ene click chemistry-based hydrogel. The results show a remarkable viability of 83% ± 4.34% and functional behaviour. Additionally, the application extends to the development of full-thickness skin constructs that exhibit characteristics of both epidermal and dermal layers. Notably, these constructs remained viable for an extended period of 41 days, underlining the potential of the integrated system in long-term tissue engineering applications.

B-BRIGHTER integrated fluorescent light sheet bioprinting and imaging system represents a significant stride forward in tissue engineering technology. Its capabilities offer researchers and healthcare professionals a powerful tool for designing and fabricating complex tissue structures with unprecedented precision and efficiency. As technology continues to evolve, the future holds exciting prospects for further advancements in light-based bioprinting, ultimately shaping the landscape of biomedical research and healthcare.


Reference article: Light Sheet-based Laser Patterning Bioprinting Produces Long-term Viable Full-thickness Skin Constructs. Levin Hafa, Louise Breideband, Lucas Ramirez Posada, Núria Torras, Elena Martinez, Ernst H.K. Stelzer, Francesco Pampaloni. https://doi.org/10.1002/adma.202306258

Last 29^th of September, researchers from 26 European countries participated in the European Researchers’ Night 2023. Among them, Elena Martínez, Marcel Sorribas and Gustaf Mårtensson presented B-BRIGHTER and talked about the science behind the project. Additionally, B-BRIGHTER was also featured on the Catalan website of the event. 

The European Researchers’ Night is a large-scale public event that takes place at the same time across various European cities. During this event, researchers present the diverse area of science and its impact on everyday life through interactive and pleasant activities. The idea behind the event is to strengthen the connection between the public and research, stimulating interest in scientific careers and research triumphs. The event also emphasizes remarkable research initiatives spanning not only Europe but also other regions. Annually, approximately 1.5 million people participate in the event, engaging with and enjoying the world of science and research.

B-BRIGHTER took part in the event in two different activities. On the 26^th of September, Elena Martínez from IBEC gave a talk about 3D Bioprinting applied to the biomedical field with the title “Bright ideas: how light allows us to create living tissues”. The audience, about 30 people from different ages, actively participated with several questions. The talk took place at the Vila Urània Civic Center, a place that brings culture at large to the society.

Finally, Gustaf Mårtensson, B-BRIGHTER’s coordinator at Mycronic, gave talks for 9^th, 10^th and 12^th graders at three schools.
 
B-BRIGHTER Project is also showcased on the Catalan website of the European Researchers’ Night, which offers information about several European projects being carried out in the region. To access this website (in English) click here.

The “Advances in 3D Bioprinting” conference, focused on the latest advancements in bioprinting methods and applications, counted with the presence of several B-BRIGHTER researchers from the Institute for Bioengineering of Catalonia (IBEC), who presented the last developments of the project.

From 10-12 September, lead international experts in 3D Bioprinting came together at Technion, Israel, to share their experience in various exciting topics in this field, including in situ bioprinting, bioprinting for organ-on-a-chip devices, organoid bioprinting, and bioprinting of vascularized composite tissues. B-BRIGHTER researchers from IBEC had the opportunity to present the latest advances of the project and in a very rich and fruitful scientific environment.

Elena Martínez was invited to give the talk “Development of biomimetic models of intestine tissue: guiding cellular self-organization through biofabrication technology”, where she emphasized the great possibilities of the 3D bioprinting technology to mimic this organ in the laboratory.

Additionally, to the invited talk, two posters + oral presentations showcased B-BRIGHTER at the conference. Aina Abad-Lázaro presented the work “A 3D model of the intestinal mucosa through light-based bioprinting combining organoids and stromal cells”, that aims to engineer an in vitro intestinal model more similar to the physiological condition, by bioprinting a villus-like 3D hydrogel with embedded stromal cells.

On the other hand, Nuria Torras was in charge of presenting the project overview, also with a poster and an oral presentation, entitled “3D Bioprinting by Light-Sheet Lithography: towards complex tissue constructs”. She highlighted the new bioprinting technology based on digital light-sheet illumination and using an original top-down lithography approach. To conclude this very successful participation, Nuria’s poster has received the conference best poster award. Congratulations!

Youth and Science program from La Pedrera Foundation in Barcelona puts together 15–16-year-old students with scientists from different fields and allow them to perform a small research project. Nuria Torras took part in this engagement activity and hosted Lola Dagà for 3 weeks at the Biomimetic systems for cell engineering group from the Institute for Bioengineering of Catalonia (IBEC).

The Catalunya La Pedrera Foundation is a private and independent foundation, led by a board of trustees comprising experts in different fields, which focus their efforts on carrying out projects that have a positive impact on people’s quality of life. Among those projects we can find the Youth and Science program, oriented to 4th year of compulsory secondary school (ESO) students. The goal of this program is to encourage student’s scientific-technological vocations offering them the opportunity to experience research firsthand and the chance of fully cultivating their skills.

For three weeks this Summer, Nuria Torras, B-BRIGHTER researcher at the Biomimetic systems for cell engineering group lead by Elena Martinez at IBEC in Barcelona, hosted a student in the frame of this program. From the 26^th of June to the 14^th of July, Lola Dagà could experience what it is like to be a researcher. This 15 years old student had a small research project entitled: 3D bioprinting of biological models: manufacturing and case study.

Among others, Lola learnt how to use light-based 3D bioprinting to manufacture several tissue models, by combining hydrogels and different cell lines, and how to prepare inks for bioprinting. Part of the training consisted in studying the effects of the geometry and various printing parameters in the model to optimize the results. Additionally, she could culture the manufactured samples and monitor cell behaviours through immunofluorescence and microscopy techniques (basic and confocal optical microscopy).

In addition to the practical work, Lola also learnt what is a scientific paper and how to find them in the literature, and how to present and discuss her work and results with team members, for example during the weekly meetings. This was a great experience for Lola who opened her mind to scientific research and developed a critical thinking. These kinds of engagement activities are very valuable to stimulate scientific vocations.

The 16th Science Party (“Festa de la Ciència” in Catalan) took place last 10 – 11 of June in Barcelona. Researchers from the Institute for Bioengineering of Catalonia proposed a hands-on activity to bring B-BRIGHTER project closer to the society.

One more year, the streets of Barcelona, Spain, filled with science and people interested in it. The 16^th edition of the Science Party was a great success and for two days, participants had the opportunity to engage in a diverse array of actions proposed. Shows, workshops, experiments, guided tours, microtalks, games, and debates composed a total of 177 activities that attracted approximately 16,000 visitors.

Researchers Nuria Torras, Marcel Sorribas and Angela Cirulli from the Biomimetic systems for cell engineering group at the Institute for Bioengineering of Catalonia (IBEC) in Barcelona were in charge of this outreach activity in the frame of B-BRIGHTER project, entitled “3D BIOimpression: how can bioengineering help solving health problems”.

Visitors could also observe under a microscope and compare samples of real human tissues with others fabricated in the laboratory by bioimpression, such as skin and intestine. They could also wear gloves and manipulate some of the materials used in the laboratory such as Petri dishes with cell cultures, tubes with nutrient solutions for cells and pipettes.

These types of outreach activities are an excellent method for bridging the gap between science and the general public, as well as for enhancing awareness of the significance of research in our daily lives and within society as a whole.

B-BRIGHTER was present at the prestigious Gordon Research Conference 2023 on “Epithelial Differentiation and Keratinization”, held last 04-09 of June in Castelldefels, Spain. The team of Ruby Shalom-Feuerstein from Technion – Israel Institute of Technology was invited to present their last advances in the research of cornea stem cells.

The Gordon Research Conference (GRC) on Epithelial Differentiation and Keratinization stands as a foremost global scientific event dedicated to propelling the boundaries of knowledge, showcasing pioneering and previously unreleased research. This year, the meeting is entitled “Novel mechanisms of regulation in epithelial biology and their implication in physiology, aging and disease”, and strives to unite distinguished speakers who are pioneers in the field of stem cell biology, cancer, regenerative medicine, immunity, aging, epigenetics, skin diseases, novel single cell molecular technologies, global genomics, and novel therapies.

Shalini began her talk by introducing B-BRIGHTER project and technology, and afterwards she focused on the use of cornea as a robust stem cell model. She emphasized its assets as it allows tracing stem cells in live with simple microscopy, observing spatial segregation of stem cells and differentiated cells, and a robust regeneration, among other advantages. To conclude her presentation, she mentioned that differentiation is a reversible process, central cornea shows high plasticity and dedifferentiated cells display bona fide stem cell features.

The Gordon Research Conference puts a strong emphasis on encouraging discussions following each presentation and cultivating casual exchanges among scientists at every stage of their careers. The conference agenda boasts a rich array of speakers and discussion leaders hailing from institutions and entities across the globe, converging to explore the most recent advancements within the domain. For sure it has been a very fruitful event for B-BRIGHTER project. 

What would happen if we could do human tissue bioprinting under 0g? This is the key question of ASTRoLAB: Advanced Space-based Tissue Regeneration with Light-sheet Assisted Bioprinting. This side-project based on B-BRIGHTER technology has been recently submitted to the European Space Agency (ESA).

B-BRIGHTER consortium members recently presented a project in the frame of the ESA Payload Master call, with the aim to send the project’s light-sheet bioprinter to space and perform some experiments to check-out if there outcomes improve while working under 0g.

The main points of the proposal are:

– Bioprinting under 0g substantially improves the quality of the engineered tissues, by allowing a level of homogeneity of the cell-gel mixture that is not achievable in a g-field 20 µm resolution.

– We want to show that manufacturing engineered tissues in 0g offers enormous advantages compared to manufacture on Earth.

– We aim to demonstrate that the top-down bio-printing process offered by the LS-Bioprinter is much more suitable to 0g applications than the conventional extrusion methods.


The ESA Payload Masters program serves the dual role of fostering and discovering groundbreaking in-orbit experiments and cutting-edge technologies for payloads. Simultaneously, it plays a pivotal role in democratizing space exploration, making it accessible to both space and non-space industries. The main objective of the program is to enhance the competitiveness of companies by eradicating the well-known “valley of death” phenomenon and giving access to in-Orbit Demonstration and Validation (IOD/IOV).

The project winners of the call will be offered the opportunity to fly their payloads/microgravity experiments on board of Nyx for the “Mission Possible” mission. Nyx is a modular and reusable orbital vehicle that can be refuelled in orbit and serves the low Earth orbit (LEO) and lunar destinations. It will start by carrying cargo, with the growth potential to fly humans afterwards. Nyx will enable to perform microgravity experiments, in-orbit technology demonstrations, entertainment, and educational missions, but also cargo delivery to larger space infrastructures or lunar surface.

Let’s keep fingers crossed and hope ASTRoLAB will be selected!